Za infection in pregnant women was confirmed by the Centers for Disease Control and Prevention survey [6]. Pregnant women have been therefore designated as a top priority group to receive the pandemic A/H1N1 2009 influenza vaccinePandemic Influenza 2009 Vaccine and Pregnancy[7?1]. In France, the Benzocaine chemical information vaccination campaign was launched in November 2009; a single dose of a non-adjuvanted A/H1N1 2009 influenza vaccine was recommended for all pregnant women 
after the first trimester [11]. Most of available data are issued from retrospective studies and prospective cohort studies are still lacking 25033180 to better understand how A/H1N1 2009 influenza pandemic affects pregnant women. Furthermore, whereas some studies 25033180 have shown safety, immunogenicity and effectiveness of seasonal flu vaccination in pregnant women [4,12,13], additional data are still needed to assess the safety and 
efficacy of maternal vaccination during pandemic period. In the context of the A/H1N1 2009 influenza pandemic, we planned a prospective study conducted in the general population of pregnant women to assess the incidence, the maternal-fetal impact of 2009 influenza pandemic, and the effectiveness and the safety of maternal vaccination. When it appeared that the pandemic level expected by public health services would be not Licochalcone-A web achieved, the objectives of the study were redefined to assess: 1) the incidence of laboratory-documented influenza 2009 pandemic, 2) the effects of pandemic vaccination on pregnancy outcome and 3) the proportion of women with seroprotection against influenza 2009 A/H1N1 at delivery, both in vaccinated and in nonvaccinated women.Patients and Methods Pandemic A/H1N1 2009 Influenza and Vaccination in FranceIn France, a first wave of A/H1N1 2009 influenza infection was reported in July 2009. Detection of pandemic A/H1N1 2009 influenza viruses remained then sporadic until week 42 (October 12?8). In the second wave, influenza like illness (ILI) incidence peaked in week 49 (November 29-December 4) and fell below the epidemic threshold in the last week of the year [14]. Pandemic A/ H1N1 2009 influenza vaccine was administered, free of charge, in centers dedicated to pandemic vaccination. A single dose of a nonadjuvanted A/H1N1 2009 influenza vaccine (PanenzaH) was recommended for all pregnant women after the first trimester of pregnancy. On November 20, 2009, PanenzaH was available and women in the second or third trimester of pregnancy asked to get vaccinated by receiving a letter from the National health insurance.Study DesignThe COFLUPREG (COhort on FLU during PREGnancy) study was a prospective cohort study conducted on pregnant women in three tertiary maternity centers in Paris (France) during the pandemic A/H1N1 2009 influenza. Women were included from October 12, 2009 to February 3, 2010. Pregnant women between 12 and 35 weeks of gestation were eligible to the study if they were aged 18 years and were speaking and understanding French. Main exclusion criteria were laboratory documented A/H1N1 2009 influenza during the last 6 months and vaccination against A/H1N1 2009 influenza before inclusion. Pregnant women were randomly selected in order to obtain a representative sample of pregnant women followed in these maternity hospitals. The draw was made on the list of women to visit the following week in each of the maternity hospitals. The randomization numbers were established using tables of order for permutation and this procedure was stratified according to cent.Za infection in pregnant women was confirmed by the Centers for Disease Control and Prevention survey [6]. Pregnant women have been therefore designated as a top priority group to receive the pandemic A/H1N1 2009 influenza vaccinePandemic Influenza 2009 Vaccine and Pregnancy[7?1]. In France, the vaccination campaign was launched in November 2009; a single dose of a non-adjuvanted A/H1N1 2009 influenza vaccine was recommended for all pregnant women after the first trimester [11]. Most of available data are issued from retrospective studies and prospective cohort studies are still lacking 25033180 to better understand how A/H1N1 2009 influenza pandemic affects pregnant women. Furthermore, whereas some studies 25033180 have shown safety, immunogenicity and effectiveness of seasonal flu vaccination in pregnant women [4,12,13], additional data are still needed to assess the safety and efficacy of maternal vaccination during pandemic period. In the context of the A/H1N1 2009 influenza pandemic, we planned a prospective study conducted in the general population of pregnant women to assess the incidence, the maternal-fetal impact of 2009 influenza pandemic, and the effectiveness and the safety of maternal vaccination. When it appeared that the pandemic level expected by public health services would be not achieved, the objectives of the study were redefined to assess: 1) the incidence of laboratory-documented influenza 2009 pandemic, 2) the effects of pandemic vaccination on pregnancy outcome and 3) the proportion of women with seroprotection against influenza 2009 A/H1N1 at delivery, both in vaccinated and in nonvaccinated women.Patients and Methods Pandemic A/H1N1 2009 Influenza and Vaccination in FranceIn France, a first wave of A/H1N1 2009 influenza infection was reported in July 2009. Detection of pandemic A/H1N1 2009 influenza viruses remained then sporadic until week 42 (October 12?8). In the second wave, influenza like illness (ILI) incidence peaked in week 49 (November 29-December 4) and fell below the epidemic threshold in the last week of the year [14]. Pandemic A/ H1N1 2009 influenza vaccine was administered, free of charge, in centers dedicated to pandemic vaccination. A single dose of a nonadjuvanted A/H1N1 2009 influenza vaccine (PanenzaH) was recommended for all pregnant women after the first trimester of pregnancy. On November 20, 2009, PanenzaH was available and women in the second or third trimester of pregnancy asked to get vaccinated by receiving a letter from the National health insurance.Study DesignThe COFLUPREG (COhort on FLU during PREGnancy) study was a prospective cohort study conducted on pregnant women in three tertiary maternity centers in Paris (France) during the pandemic A/H1N1 2009 influenza. Women were included from October 12, 2009 to February 3, 2010. Pregnant women between 12 and 35 weeks of gestation were eligible to the study if they were aged 18 years and were speaking and understanding French. Main exclusion criteria were laboratory documented A/H1N1 2009 influenza during the last 6 months and vaccination against A/H1N1 2009 influenza before inclusion. Pregnant women were randomly selected in order to obtain a representative sample of pregnant women followed in these maternity hospitals. The draw was made on the list of women to visit the following week in each of the maternity hospitals. The randomization numbers were established using tables of order for permutation and this procedure was stratified according to cent.
