Name :
Biotinylated Human VEGF121 Protein, Avitag™,His Tag
Background :
Vascular endothelial growth factor (VEGF) is also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87% aa sequence identity with corresponding regions of mouse and rat, 93% with feline, equine and bovine, and 91%, 95% and 96% with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.
Biological Activity :
Immobilized VEGFR1/R2-Fc at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF121, Avitag,His Tag (Cat. No. VE1-H82E7) with a linear range of 0.1-2 ng/mL (QC tested).
Species :
Source :
MABSol® Biotinylated Human VEGF121, Avitag,His Tag (VE1-H82E7) is expressed from human HEK293 cells. It contains AA Ala 27 – Arg 147 (Accession # P15692-9 ).
Tag :
Synonyms :
（Synonym）RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
Purity :
（Purity）>95% as determined by SDS-PAGE.
Storage and Stability :
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin Level :
（Endotoxin）Less than 1.0 EU per μg by the LAL method.
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Protein Structure :
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag
Refactoring Approach :
Please see Certificate of Analysis for specific instructions.
Protein Labeling :
Passed as determined by the HABA assay / binding ELISA.
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