Name :
SEMA3A Protein
Description :
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer’s disease
Species :
Mouse
Uniprotkb :
HEK293
Tag :
His
Synonyms :
SemD, Semad, Hsema-I, SEMA1, coll-1, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Construction :
A DNA sequence encoding the mouse SEMA3A (NP_001230001.1) (Lys26-Phe546) was expressed with a polyhistidine tag at the N-terminus.
Protein Purity :
> 95 % as determined by SDS-PAGE.
Molecular Weight :
Approxiamtely 61.6 kDa
Endotoxin :
Formulatione :
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, 100 mM Argine, 100 mM glutamic acid, pH 8.5. Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
Semaphorins are a family of secreted and cell-bound signaling molecules defined by the presence of a common 5 aa Sema domain. They are best characterized in relation to axon guidance during development of the nervous system. The functions of Semaphorins 3A (SEMA3A) are mediated primarily through binding to the Neuropilin-1 (Npn-1) and Plexin-A1 coreceptor complex. Neuropilins lack a signaling-competent cytoplasetmic domain and ensure semaphorin binding, whereas the transmembrane receptor plexin mediates the intracellular response. As the first identified vertebrate semaphorin, SEMA3A functions either as a chemorepulsive agent inhibiting axonal outgrowth, or as a chemoattractive agent stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Its overexpression is associated with schizophrenia which is seen in various human tumor cell lines, and aberrant release is associated with the progression of Alzheimer’s disease
References and Literature :
1. Giordano,A. et al., 2003, J Neurocytol.32(4):345-352. 2. Good, P. F. et al., 2005, J. Neurochem.91(3): 716-736. 3. Gu, C. et al., 2005, Science.307(5707): 265–268. 4. Chadborn,N.H. et al., 2006, J Cell Sci.119(Pt 5):951-957. 5. Schmidt,E.F. et al., 2007, Adv Exp Med Biol.600:1-11.
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