In this study, Professor Zhengbao Zha and Tao He’s research groups at Hefei University of Technology have developed a novel class of aggregation-induced emission (AIE) gen-based microbubbles (AIE-Gd MBs) designed for ultrasound-lit-up fluorescence imaging in surgical navigation. This innovative strategy leverages the unique properties of AIEgens—fluorophores that emit strong light when aggregated—to overcome the limitations of traditional fluorophores, which suffer from aggregation-caused quenching (ACQ). By integrating AIEgens with gadolinium chelates into an amphipathic polymer backbone, the researchers created a multifunctional contrast agent capable of tri-modal imaging: ultrasound (US), magnetic resonance imaging (MRI), and fluorescence imaging (FI).SMN1 Antibody custom synthesis
The core innovation lies in the use of ultrasound-responsive microbubbles. Upon intravenous injection, AIE-Gd MBs circulate through the bloodstream and accumulate in tumor tissues via the enhanced permeability and retention (EPR) effect. When targeted ultrasound is applied, the microbubbles undergo ultrasound-targeted microbubble destruction (UTMD), leading to cavitation and a rapid conversion from microbubbles to nanoparticles (NPs). This transformation significantly enhances the aggregation of AIEgen molecules within the NPs, thereby activating their fluorescence emission. The resulting “light-up” effect provides real-time, high-sensitivity fluorescence signals that can guide surgeons during tumor resection.
The system demonstrates excellent performance across multiple imaging modalities. Preoperative MRI and US imaging clearly outline tumor boundaries and size, while intraoperative FI offers real-time visualization of tumor margins, reducing the risk of residual cancer tissue.HNRNPF Antibody manufacturer Postoperative MRI further confirms complete tumor removal by detecting any remaining AIE-Gd signal.PMID:35023011 In vivo experiments using 4T1 tumor-bearing mice confirmed effective tumor targeting, strong fluorescence activation upon ultrasound exposure, and no significant toxicity. Biocompatibility assessments including MTT assays, hemolysis tests, and histopathological analysis revealed no adverse effects on vital organs or systemic health.
This work presents a transformative approach to surgical navigation by combining non-invasive imaging guidance with real-time fluorescent feedback. The ability to “light up” AIEgens precisely at the tumor site using ultrasound not only improves accuracy but also simplifies the workflow for surgeons. With its potential for clinical translation, this tri-modal platform represents a significant advancement in precision oncology, offering a powerful tool to enhance surgical outcomes and patient survival.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
