Product Name :
Toxin A (Clostridium difficile)
Sequence:
Purity:
Molecular Weight:
~270kDa
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Use/Stability :
As indicated on product label or CoA when stored as recommended. Stable for 2 months at +4°C after reconstitution in sterile water at a concentration of 0.2 mg/ml.
Description:
Cytotoxic and enterotoxic Has potent cytotoxic and enterotoxic properties. Translocates to the cytosol of target cells and glucosylates small GTP-binding proteins such as Rho, Rac, and Cdc42. Induces actin condensation, cell death and opening of the tight junctions.23109-05-9 custom synthesis Triggers release of pro-inflammatory cytokines from intestinal epithelial cells probably via activation of MAP kinases.220619-73-8 Purity & Documentation
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Solubility:
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Additional Information :
| Activity Each lot is tested to confirm binding activity to fresh rabbit red blood cells using a hemagglutination assay.PMID:31151954 The lowest concentration producing a positive reaction was ~50µg/ml. | Formulation Lyophilized from 50mM TRIS, pH 7.5, containing 50mM sodium chloride and 0.1% trehalose. | MW ~270kDa | Purity Detail When examined on 3-8% SDS-PAGE gels, this protein migrates as a single major band with an apparent molecular weight of 300,000 Dan run. Densitometric analysis estimates the purity of the product as >80%. | Reconstitution Reconstitute with sterile distilled water. | Source Isolated from Clostridium difficile. | Technical Info / Product Notes Protein concentration is determined by the Bradford method using BSA as a standard. | UniProt ID P16154
