Cosins A and B (regioisomers arisingFrontiers in Plant Science www.frontiersin.orgNovember Volume ArticleSisTerraza et al.Coumarins in FeDeficient Arabidopsis PlantsTABLE MSMS information for some of the compounds secreted and accumulated by Arabidopsis thaliana roots in response to Fe deficiency mz ratios of the fragment ions and their relative intensity.Compound # Annotation Parent ion mz ..Ion type ESIMSn mz (Relative intensity, in )Coniferylaldehyde hexoside ,dihydroxymethoxycoumarin hexoside (fraxetin hexoside) hydroxy,dimethoxycoumarin hexoside (isofraxidin hexoside)[MH] [MH]MS , , , , , , MS MS , , , , , , MS MS , , , , , , , , , , , , , , , , , , , , , , , , , , MS MS , , , , , , , , , , , , , MS MS , , MS , , , , MS , , , , , , .[MH]Sinapyl aldehyde hexoside.[MH] Ferulic acid Coniferyl aldehyde Sinapyl aldehyde…[MH] [MH] [MH]Numbers in italics (Compound #) refer to the labels made use of for each and every compound in Table .All information had been taken in the HPLCESIMSMS(ion trap) evaluation.The important ion of the MS and MS spectra is also indicated in bold.in the fusion of fraxetin as well as the monolignol hydroxyconiferyl alcohol, Cheng and Chen, , Figure C), possess a formula identical to that of compound (C H O).The structural variations among these coumarinolignans corresponding for the monolignol moiety (Figure B) are identical to these discovered amongst the elemental formulae of (i) a methoxy group differentiates coniferyl from sinapyl Trisodium citrate dihydrate In Vitro alcohols and the elemental formula of from that of ; (ii) a hydroxyl group differentiates hydroxyconiferyl from coniferyl alcohols and also the elemental formula of from that of ; and (iii) a methyl group differentiates hydroxyconiferyl and sinapyl alcohols as well as the formula of from these of .To confirm the identification of as cleomiscosins, we isolated coumarinolignans from C.viscosa seeds.The seed isolate was analyzed by both HPLCUVVISESIMS(TOF) and HPLCESIMS(ion trap) applying Elution plan and positive ESI ionization.The HPLCESIMS(TOF) chromatogram for mz . corresponding towards the cleomiscosins C and D [MH] ions, showed only two peaks, at .and .min, matching with all the RTs of and (Figure C; Table).Similarly, the HPLCESIMS(TOF) chromatogram for mz . corresponding to the cleomiscosins A and B [MH] ions, showed only two peaks, at .and .min, matching with all the RTs of (Figure C; Table).Peaks were assigned to cleomiscosin isomers as outlined by the elution order reported inside the literature (Chattopadhyay et al Kaur et al).These annotations had been confirmed by the complete match between the MS spectra of your cleomiscosins D, C, B, as well as a, and those of compounds , , and , respectively (Figure C).Compound eluted at shorter instances than the cleomiscosins (.vs..min), as expected in the structural differences in between hydroxycleomiscosin A and B and cleomiscosins (see above).In addition, compound shares elemental formula plus the presence of a fraxetin moietywith hydroxycleomiscosins A and B, and its MS spectrum showed a loss of Da from the [MH] ion (Figure B; Supplementary Table S), which was previously reported for hydroxycleomiscosin A (Cheng and Chen,) but doesn’t happen in cleomiscosins.For that reason, was putatively annotated as hydroxycleomiscosin A andor B (Table ).Coumarin and Coumarinolignan Concentrations in Root ExtractsQuantification of phenolic compounds was carried out using the PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21541725 [MH] and [MhexosideH] signals inside the HPLCESIMS(TOF).Coumarin.
