Comprehend the mechanisms of relapse. This technologies is still experimental, yet it has sparked substantially interest inside the scientific community mainly because it promises a brand new era of cancer management. We here review its application within a subset of tumors characterized by the presence of your ALK oncogene: individuals affected by these tumors can benefit from targeted therapy, but show frequent relapses, which call for improved solutions of disease detection. Abstract: Cancer cells are characterized by higher genetic instability, that favors tumor relapse. The identification in the genetic causes of relapse can direct next-line therapeutic alternatives. As tumor tissue rebiopsy at disease progression is just not normally feasible, noninvasive option techniques are getting explored. Liquid biopsy is emerging as a non-invasive, simple and repeatable tool to recognize particular molecular alterations and monitor disease response throughout therapy. The dynamic follow-up offered by this analysis can offer useful predictive details and let prompt therapeutic actions, tailored for the genetic profile in the recurring disease, several months before radiographic relapse. Oncogenic fusion genes are especially suited for this kind of analysis. Anaplastic Lymphoma Kinase (ALK) is the dominant driver oncogene in quite a few tumors, like Anaplastic Large-Cell Lymphoma (ALCL), Non-Small Cell Lung Cancer (NSCLC) and other folks. Here we overview current findings in liquid biopsy technologies, including ctDNA, CTCs, exosomes, as well as other markers which can be investigated from plasma samples, in ALK-positive cancers. Keyword phrases: ALK; lung cancer; liquid biopsyCitation: Villa, M.; Sharma, G.G.; Manfroni, C.; Cortinovis, D.; Mologni, L. New Advances in Liquid Biopsy Technologies for Anaplastic Lymphoma Kinase (ALK)–Positive Cancer. Cancers 2021, 13, 5149. https://doi.org/10.3390/cancers13205149 Academic Editor: Samuel C. Mok Received: 7 September 2021 Accepted: 11 October 2021 Published: 14 OctoberPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.1. Introduction Cancer is a clonal illness characterized by the evolution of heterogeneous subpopulations that follow Darwinian processes of choice. In comparison to standard species evolution, tumors show fast Ferrous bisglycinate adaptation towards the environment, as a consequence of their inherent genetic instability and big population size. Next-generation sequencing (NGS) technologies have revolutionized our ability to analyze cancer genetic diversity. From pioneering multi-region sequencing research to current single-cell analyses, the accumulated data point to high intra-tumor heterogeneity, which poses significant challenges to therapies: tumors Stearic acid-d3 site continue to evolve beneath treatment and often adapt to a brand new environment represented by therapies. Below these circumstances, uncommon clones that are resistant to drugs will emerge due to the evolutionary pressure exerted by the treatment. Genetic evolution also can shape the seeding of distant metastases, through population bottlenecks and the acquisition (choice) of new attributes that confer the capacity to colonize diverse habitats. It has beenCopyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This short article is definitely an open access article distributed under the terms and circumstances on the Inventive Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).Cancers 2021, 13, 5149. https://doi.org/10.3390/cancershttps://www.mdpi.co.