Ca and 52.0 mg for a. cryptum (no matter the addition of Cu2 ). For the Cr(VI) reduction test, 1.0 mg of bio-Pt(0)NPs (equivalent to 0.22 mg of net-Pt(0) for Ac. aromatica and 0.19 mg of net-Pt(0) for a. cryptum) was resuspended into 20 mL of fresh HBS medium (pH 2.5) inside a 20 mL vial bottle. For comparison, Pt bulk powder (10 , PX-478 Autophagy Sigma-Aldrich, Tokyo, Japan: 327476) and Pt/C (ten wt. loading, matrix-activated carbon support, Sigma-Aldrich, Tokyo, Japan: 205958)3-Chloro-5-hydroxybenzoic acid medchemexpress Minerals 2021, 11,weight of theof the freeze-dried bio-Pt(0)NPs44.four mg for Ac. aromatica and 52.0 mg for any.for any. weight freeze-dried bio-Pt(0)NPs was was 44.4 mg for Ac. aromatica and 52.0 mg cryptum (regardless of the addition of Cuof).Cu2). For the Cr(VI) reduction 1.0 mg of bio- biocryptum (no matter the addition two For the Cr(VI) reduction test, test, 1.0 mg of Pt(0)NPs (equivalent to 0.22 mg of net-Pt(0) for Ac. aromatica and 0.19 mg of net-Pt(0) for for Pt(0)NPs (equivalent to 0.22 mg of net-Pt(0) for Ac. aromatica and 0.19 mg of net-Pt(0) A. cryptum) was resuspended into 20 mL of fresh fresh medium (pH two.five) in a 20 mL vial vial A. cryptum) was resuspended into 20 mL of HBS HBS medium (pH 2.five) within a 20 mL bottle. For comparison, Pt bulk powder (10 , Sigma-Aldrich, Tokyo, Japan: 327476) bottle. For comparison, Pt bulk powder (ten m, Sigma-Aldrich, Tokyo, Japan: 327476) 4 of 11 and Pt/C Pt/Cwt. wt. loading, matrix-activated carbon help, Sigma-Aldrich, Tokyo, and (ten (10 loading, matrix-activated carbon assistance, Sigma-Aldrich, Tokyo, Japan: 205958) had been weretested (0.two mg of net-Pt(0) was utilised). Cr(VI) (as Na2CrO42H2O) 2O) Japan: 205958) also also tested (0.2 mg of net-Pt(0) was utilized). Cr(VI) (as Na CrO44H and formate (as an electron donor) were have been added at 10 mg/Lmg/L10 mM, respectively. then then added at 10 and and 10 mM, respectively. and formate (as an electron donor) All options had been had been anaerobicallywas made use of). by purging N2(1 mg/L2 O) and formate vial All solutions anaerobically prepared by purging (as Na gas H DO). The The have been also tested (0.2 mg of net-Pt(0) prepared Cr(VI) N2 gas 2CrO4(1 mg/L DO). vial bottles have been had been sealed butylbutyl rubber stoppers and incubated unshaken . Samples (as an electron donor) had been then added at ten mg/L and ten mM, respectively. at 30 . Samples bottles sealed with with rubber stoppers and incubated unshaken at 30 All solutions had been had been withdrawn periodically to monitor Cr(VI) concentrationsbottles were sealed werewithdrawn periodically to monitor2 gas (1 mg/L DO). The vial (diphenyl carbazide anaerobically ready by purging N Cr(VI) concentrations (diphenyl carbazide method [24]). [24]). stoppers and incubated unshaken at 30 C. Samples were withdrawn with butyl rubber strategy periodically to monitor Cr(VI) concentrations (diphenyl carbazide technique [24]). three. Outcomes and Discussion 3. Benefits and Discussion 3. Impact of and Discussion 3.1. Benefits Pt(IV) on Bacterial Cell Development 3.1. Impact of Pt(IV) on Bacterial Cell Growth three.1. Impact of Pt(IV) on Bacterial Cell Growth For the Ac. aromatica growth, the presence of 0.5of 0.50.75 mg/Lmg/L of Pt(IV) increasingly For the Ac. aromatica development, the presence and and 0.75 of Pt(IV) increasingly For the lag-phase, but the final cell the presence of was comparable of your case without extended the Ac. aromatica growth, the finaldensity0.5 and 0.75 mg/L to Pt(IV) increasingly extended the lag-phase, but cell density was comparable for the case without the need of extended the9lag-phase, but.
