group than in the T0 group. Adding curcumin in diet regime significantly decreased TBIL level (p = 0.043) inside the T500 + AFB1 group with respect for the T0 + AFB1 group. As expected, there was no c-Rel custom synthesis important difference in TBIL level between the T500 + AFB1 group and T0 group (p 0.05) (Figure 1E). No substantial difference in ALP (p = 0.621) and also a decreasing trend in ALP (p = 0.676) were HSP70 Gene ID observed amongst groups (Figure 1F). There was no substantial boost in ALT (p = 0.246) and AST (p = 0.065) activity within the T0 + AFB1 group relative to these in the T0 group. Adding curcumin into diet regime inhibited the activities of ALT (p = 0.544) and AST (p = 0.140) within the T500 + AFB1 group relative to these in the T0 + AFB1 group, but with no important variations. No considerable difference in ALT and AST activity among the T0 + AFB1 group along with the T0 group was located (p 0.05) (Figure 1G,H). three.2. Evaluation of Pathological Sections and Ultrastructural Assessment in Liver Histopathological examination of H E-stained livers shown in Figure 2. Within the T0 group, hepatocytes morphology was regular (Figure 2A). AFB1 administration brought on clear toxicity containing vacuolation of hepatocytes, swelling of hepatocytes, and inflammatory cell infiltration in the T0 + AFB1 group compared to the T0 group (Figure 2B). Dietary curcumin protected the liver against harm through the decrease in the quantity of inflammatory cells and swelling of hepatocytes within the liver of ducks in the T500 + AFB1 group compared with inside the T0 + AFB1 group (Figure 2C). A few inflammatory cells and swelling of hepatocytes inside the T500 + AFB1 group compared with all the T0 group was noticed. The results of this study demonstrate that dietary curcumin could shield duck liver against acute harm induced by AFB1 administration. The liver ultrastructure is shown in Figure 2. Within the T0 group, the cell nucleus and mitochondrial ridge of hepatocytes had been clearly visible as well as the chromatin in the cell nucleus was evenly distributed (Figure 2D). In comparison using the T0 group, the hepatocyte nucleus was visibly deformed; chromatin was aggregated plus the hepatocyte mitochondrial ridge was enlarged and deformed inside the T0 + AFB1 group (Figure 2E). As expected, in comparison with all the T0 + AFB1 group, hepatocyte nucleus and mitochondrial ridge have been clearly visible as well as the chromatin aggregation of hepatocytes was observed inside the T500 + AFB1 group (Figure 2F). Furthermore,Foods 2021, ten,5 ofFoods 2021, ten, x FOR PEER Evaluation the5 the hepatocyte nucleus and mitochondrial ridge had been clearly visible when comparing of 19 T500 + AFB1 group and T0 group.Figure 1. The plasma biochemical levels of ducks exposed to AFB1 at 12 h. (A) The TP content inside the Figure 1. The plasma biochemical levels of ducks exposed to AFB1 at 12 h. (A) The TP content inside the plasmaof ducks; (B) The ALB content inin the plasma of ducks; (C) The GLO contentthe the plasma plasma of ducks; (B) The ALB content material the plasma of ducks; (C) The GLO content material in in plasma of of ducks; (D) The price of ALB/GLO; (E) The TBIL activity inside the plasma of ducks; (F) The ALP acducks; (D) The rate of ALB/GLO; (E) The TBIL activity within the plasma of ducks; (F) The ALP activity tivity inside the plasma of ducks; (G) The ALT activity inside the plasma of ducks; (H) The AST activity in inside the plasma of ducks; (G) The ALT activity within the plasma of ducks; (H) The AST activity in the the plasma of ducks; (I) The price of AST/ALT. Values imply the mean SEM (common error (SE) of Foods 2021,
