Ng default parameters and 1000 bootstraps with RAxML v8.2.12 [49]. The 16s rRNA
Ng default parameters and 1000 bootstraps with RAxML v8.2.12 [49]. The 16s rRNA gene of Staphylococcus aureus (RefSeq ID: GCF_000013425.1) was used as an outgroup. The origin of Cytochrome P450 Inhibitor supplier replication (OriC) was identified making use of DoriC database [50] and Mauve aligner [51]. Pairwise genomic comparison of strain BSE6.1 was produced with 3 other Factor Xa list associated genomes. Dotplots have been constructed with minimap2 based pairwise alignment making use of D-Genies [52]. Prokka v1.14.6 was utilised to perform a regional de novo annotation [53]. Pan-genome comparison with one hundred associated genomes ( 90 16S nucleotide identity; 80 whole-genome aligned fraction identity) was made utilizing the pan-genome tool at KBase server [46]. Gene clusters associated for the secondary metabolite biosynthesis had been identified utilizing the antiSMASH 5.0 pipeline [54]. The red pigmentproducing gene cluster of BSE6.1 was compared with that of S. coelicolor A3(2), Serratia, and Hahella using the multigene BLAST tool [55]. The distribution of numerous coding sequences (CDS) and gene clusters across the genome was plotted applying Circos [56].Microorganisms 2021, 9, x FOR PEER REVIEW4 ofMicroorganisms 2021, 9,A3(2), Serratia, and Hahella utilizing the multigene BLAST tool [55]. The distribution17 vari4 of of ous coding sequences (CDS) and gene clusters across the genome was plotted making use of Circos [56].Figure 1. Workflow and pipeline of toolsand pipeline of tools used reads into a genome reads into a genome and additional Figure 1. Workflow utilized to assemble the raw to assemble the raw and further analysis of the assembled genome. analysis of the assembled genome.three. Final results and Discussion Strain BSE6.1 produced a pink-colored growth in Minimal broth with 2 NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with whiteMicroorganisms 2021, 9, x FOR PEER REVIEW5 of3. Results and DiscussionMicroorganisms 2021, 9,Strain BSE6.1 produced a pink-colored growth in Minimal broth with two NaCl and red pigmentation in all other compatible media. Pale pink to reddish colonies with white powdery spores were observed following 7 or ten days of incubation. Salt tolerance was observed as much as a rangeobserved right after 7 orbacterium incubation. Salt tolerance was observed powdery spores had been of two to 7 . This 10 days of was constructive for catalase and oxidase activities. In our earlier study, strain BSE6.1 showed possible antibacterial activity against as much as a range of 2 to 7 . This bacterium was positive for catalase and oxidase activities. different human pathogens and also displayed a powerful capability toactivity against unique In our earlier study, strain BSE6.1 showed possible antibacterial stain epidermis and parenchyma cells of Tridax procumbens stem [25]. The maximum pigmentand parenchyma human pathogens as well as displayed a sturdy capability to stain epidermis production was observed at 29procumbens stem [25]. The maximum pigmentfor its development was 38 (Figcells of Tridax , and also the maximum temperature tolerance production was observed at ure2). and also the maximum temperature with the red for its development was 38 Cobserved2). The 29 C, The peak absorption spectrum tolerance pigment of BSE6.1 was (Figure at 528 nm [25]. peak absorption spectrum of your red pigment of BSE6.1 was observed at 528 nm [25].5 ofFigure Morphological and biochemical Figure two. Morphological and biochemical traits of Streptomyces sp. strain BSE6.1.Identification of your red pigment via thin layer chromatography (TLC), FourierIdentification on the red.
