present in 250300pixel. We regarded as that the interaction amongst MP andMContdoi: ten.3164/jcbn.20-197 021 JCBNCPAlABCIntensity (arb. units)CPA CPM+MP MPDEIntensity (arb. units)CPA CPM+MP MPDistance (pixel)Distance (pixel) CPA CPM+MP MPCPA CPM+MP MPFig. 6.CPACPM+MP MPTLC assay. (A) The vibrant field image. The extraction of CPA (B) and its line profile (C). The extraction of MP (D) and its line profile (C).CPA can make some cytotoxicity compounds or accelerate intracellular CPA accumulation. We reported the antioxidant impact of MP. MP induce cancer particular apoptosis by means of accumulation of ceramide.(16) This directly cell death was induced reduction of ROS production. On the other hands, cells were exposed 200g/ml MP within this study. This concentration did not show the cytotoxicity, however the cytotox icity of CPA was enhanced. We demonstrated that antioxidant can boost the cytotoxicity of anticancer drugs indirectly. We have already reported that antioxidants therapies upregulate
Heliyon 7 (2021) eContents lists out there at ScienceDirectHeliyonjournal homepage: cell/heliyonReview articleThe correlation amongst the amount of 3-hydroxypropyl mercapturic acid, CYP2B6 polymorphisms, and hematuria occurrences following cyclophosphamide administration and its bioanalytical strategies: A systematic reviewYahdiana Harahap a, b, , Farhan Nurahman a, Denni Joko Purwanto b, Arry Yanuar aa bFaculty of Pharmacy, Universitas Indonesia, Depok, 16424, West Java, Indonesia Faculty of Military Pharmacy, Indonesia Defense University, Bogor, West Java, IndonesiaA R T I C L E I N F OKeywords: Cyclophosphamide (CPA) 3-hydroxypropyl mercapturic acid (3-HPMA) CYP2B6 Hemorrhagic cystitis HematuriaA B S T R A C TBackground: Cyclophosphamide (CPA) can be a cytotoxic prodrug that requirements to become metabolized by cytochrome P450 enzymes, like CYP2B6. Unfortunately, CYP2B6 can be a pretty polymorphic enzyme and can bring about a MT1 custom synthesis transform in 3-hydroxypropyl mercapturic acid (3-HPMA), by far the most found CYP metabolite in urine levels. Change in 3-HPMA levels may also indicate the level alter in its precursor, acrolein, that is accountable for the hematuria incidence right after CPA administration. This review’s goal is to obtain a conclusion concerning the optimal 3-HPMA analysis system in urine after the administration of cyclophosphamide working with liquid chromatography-tandem mass spectrometry (LC-MS/MS) via literature overview from prior studies. Also, this overview was written to examine the connection amongst levels of 3-HPMA in urine, polymorphisms of CYP2B6 enzymes, and also the incidence of hematuria following cyclophosphamide administration in cancer patients. Techniques: Significant databases, which include Universitas Indonesia’s library database ScienceDirect, PubMed/Medline, Frontiers Media, and Google Scholar database, have been used to discover both published and unpublished research without a time limit until 2020. Studies on pharmacokinetics, pharmacodynamics, drug therapy monitoring of cyclophosphamide, bioanalysis, and polymerase chain reaction (PCR) published in Indonesian and English were integrated. Meanwhile, non-related studies or research written in other languages in addition to Indonesian and English had been excluded. Two independent reviewers screened the titles, abstracts, and PDE1 Species full-text manuscripts. Data obtained from eligible sources had been utilized to answer the purpose of this assessment inside a narrative form. Outcomes: The authors found 436 connected studies from several databases and web-sites. Then, the authors narrowed it down into
