He experiment plus the extract was administered as single dose and
He experiment along with the extract was administered as single dose and observed for the mortality up to 48 h study period (quick term toxicity). Determined by the short term toxicity profile, the next dose of your extract was determined as per OECD suggestions No.420. The maximum dose tested (2000 mg/kg) for LD50. In the LD50, doses like 1/20th, 1/10th and 1/5th have been selected and deemed as low, medium and higher dose i.e., 100 mg/kg, 200 mg/kg, 400 mg/kg respectively to carry out this study.Experimental DesignThe diuretic activity of alcoholic extract of roots of IL-2 site Cissampelos pareira in albino rats was studied by the Lipschitz Test [16-18]. Male Albino rats had been divided into 5 groups of six rats in every single. The group I serves as normal handle received automobile (CMC two in normal saline 10 ml/kg b.wt), the group II received Furosemide (ten mg/kg, p.o) in vehicle; other groups III, IV, V had been treated with low, medium, and higher doses of alcoholic extract of roots of Cissampelos pareira in car and quickly just after the extract treatment all of the rats had been hydrated with saline (15 ml/kg) and placed within the metabolic cages (2 per cage), specially created to separate urine and faeces andS. no. 1 2 three 4 5 groups Handle (ten ml/Kg b. wt) Common (Frusemide 10 mg/kg b.wt) Alcoholic extract of roots of C.pareira Low (100 mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira High (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals offer a natural safeguard against ailments and are a substantial remedy for specific illnesses. CECR2 review Diuretics have proved to be particularly valuable in the treatment of mild to moderate hypertension and also in enhancing the impact of other antihypertensive agents. Diuretics relieve pulmonary congestion and peripheral oedema. These agents are useful in lowering volume over load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They decrease plasma volume and subsequently venous return to the heart. This decreases the cardiac function load, oxygen demand and plasma volume and also decreases blood pressure. Thusna+ mmol/l 113.03 + 2.16 191.05+2.09 129.40+2.*** ***total urine Vol (ml/kg b.wt/5 h) 13.45.02 22.23.01 15.20.*** ***K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1.*** ***Cl- mmol/l 82.95 + 1.42 129.06+1.67*** 94.42 + 1.73*** 109.44+1.20*** 121.39+2.00***17.41.02*** 20.46.***164.99+2.00*** 184.53+2.***77.93+2.67*** 85.11+1.***[Table/Fig-1]: Impact of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as mean S.E.M.,n=6, Significance at p0.05*, p0.01**, p0.001***, Compared with control group (One particular Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Analysis. 2014 Might, Vol-8(five): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in distinctive plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with most of these plant phytochemical substances described above. Hence it might be reported that the observed diuretic activity is as a consequence of these above phytoconstituents.CONCLUSIONResults showed that single dos.
