Ccuracy RE( ) 3.85 -0.73 -0.18 -4.02 0.89 1.26 -2.61 -1.81 -2.07 Precision RSD( ) 6.04 1.24 2.02 three.76 four.21 3.93 two.05 3.01 two.11 Measured (mean sirtuininhibitorSD) 0.64 sirtuininhibitor0.08 11.82 sirtuininhibitor0.52 475.six sirtuininhibitor4.11 0.78 sirtuininhibitor0.04 15.94 sirtuininhibitor0.27 498.four sirtuininhibitor3.08 0.82 sirtuininhibitor0.06 15.94 sirtuininhibitor0.20 501.6 sirtuininhibitor3.26 Inter-day Accuracy RE( ) 6.11 -2.03 -1.08 two.15 -1.09 -0.56 1.04 -2.59 -1.02 Precision RSD( ) 5.60 7.58 3.26 4.08 eight.13 2.12 4.65 7.18 3.Table 4: The extraction recovery and matrix impact in the analytes in rat plasma at low, medium, high concentration levels (n = 6) Analytes Berberine Jatrorrhizine Palmatine Concentration (ng/mL) 0.60 12.0 480.0 0.80 16.0 500.0 0.80 16.0 500.0 Extraction recovery (imply sirtuininhibitorSD, ) 95.86 sirtuininhibitor8.12 90.92 sirtuininhibitor3.04 90.71 sirtuininhibitor3.56 95.53 sirtuininhibitor7.15 97.72 sirtuininhibitor6.58 98.70 sirtuininhibitor2.05 90.13 sirtuininhibitor9.03 91.32 sirtuininhibitor2.68 93.63 sirtuininhibitor3.26 RSD ( ) 6.36 five.15 4.72 10.02 five.03 6.21 8.02 four.08 eight.42 matrix impact (mean sirtuininhibitorSD, ) 109.ten sirtuininhibitor2.06 93.84 sirtuininhibitor3.52 98.58 sirtuininhibitor3.01 104.29 sirtuininhibitor5.15 94.67 sirtuininhibitor2.IFN-beta Protein Formulation 42 100.TARC/CCL17 Protein Synonyms 81 sirtuininhibitor8.10 110.75 sirtuininhibitor3.03 109.81 sirtuininhibitor1.92 109.34 sirtuininhibitor4.16 RSD ( ) 3.36 3.08 5.12 4.78 1.93 four.12 two.43 three.00 four.Pharmacognosy Magazine, JanuaryMarch 2017, Vol 13, IssueYUAN ZI-MIN, et al.: Comparative Pharmacokinetic Between Raw and Bile-processed Rhizoma coptidisTable 5: The stability of your analytes in rat plasma (n = six) Analytes Concentration (ng/mL) Berberine 0.PMID:24238102 60 12.0 480.0 Jatrorrhizine 0.80 16.0 500.0 Palmatine 0.80 16.0 500.0 At room temperature for 12h Imply sirtuininhibitorSD ng/mL 0.58 sirtuininhibitor0.03 11.97 sirtuininhibitor0.23 493.61 sirtuininhibitor3.05 0.82 sirtuininhibitor0.13 15.67 sirtuininhibitor0.41 495.24 sirtuininhibitor5.62 0.78 sirtuininhibitor0.03 16.11 sirtuininhibitor0.23 492.03 sirtuininhibitor5.05 RE three.08 7.71 8.02 three.22 -5.56 four.21 2.42 -3.71 6.12 At -80 for 30 days Mean sirtuininhibitorSD ng/mL 0.58 sirtuininhibitor0.03 12.86 sirtuininhibitor1.26 482.04 sirtuininhibitor1.82 0.88 sirtuininhibitor0.06 16.24 sirtuininhibitor0.23 516.02 sirtuininhibitor3.65 0.85 sirtuininhibitor0.06 15.97 sirtuininhibitor0.23 495.12 sirtuininhibitor5.08 RE 5.12 eight.12 -5.36 four.05 -5.21 six.02 -1.22 -5.11 -3.82 Just after three freeze-thaw cycles Mean sirtuininhibitorSD ng/mL 0.58 sirtuininhibitor0.021 11.81 sirtuininhibitor1.26 472.26 sirtuininhibitor6.81 0.75 sirtuininhibitor0.14 15.82 sirtuininhibitor0.43 493.13 sirtuininhibitor4.65 0.76 sirtuininhibitor0.08 16.21 sirtuininhibitor0.32 494.01 sirtuininhibitor8.05 RE three.16 -5.62 -6.07 -2.20 -4.28 -6.02 2.04 7.01 -8.03 Post-treatment for 24h at 4 Mean sirtuininhibitorSD ng/mL 0.58 sirtuininhibitor0.03 11.85 sirtuininhibitor0.26 486.03 sirtuininhibitor3.62 0.84 sirtuininhibitor0.15 16.36 sirtuininhibitor0.18 492.11 sirtuininhibitor5.05 0.83 sirtuininhibitor0.03 15.82 sirtuininhibitor0.42 496.61 sirtuininhibitor3.25 RE three.16 5.14 five.05 3.26 four.22 -3.02 1.05 -2.78 4.Table six: The pharmacokinetic parameters of the three analytes in rats soon after the oral administration of Rhizoma Coptidis and pigs’ bile processed Rhizoma Coptidis extract (n = eight) Analytes Berberine Jatrorrhizine Palmatine psirtuininhibitor0.05, vs RC group Extract RC BRC RC BRC RC BRC AUC0.
