From An-Nan Hospital, China Healthcare University, Tainan, Taiwan; CMRC-CMA-2 from Greater Education Sprout Project by the Ministry of Education (MOE), Taiwan; Chinese Medicine Analysis Center, China Health-related University in the Featured Regions Research Center ProgramJ. Clin. Med. 2022, 11,13 ofwithin the framework of your Larger Education Sprout Project by the Ministry of Education (MOE) in Taiwan (CMRC-CMA-6); CMU108-SR-106 and CMU110-SR-73 from the China Medical University, Taichung, Taiwan; and CMU104-S-16-01, CMU103-BC-4-1, CMU110-N-17, CRS-108-048, DMR-102076, DMR-103-084, DMR-106-225, DMR-107-204, DMR-108-216, DMR-109-102, DMR-109-244, DMRHHC-109-11 and DMR-HHC-109-12, DMR-HHC-110-10, DMR-110-124 from the China Medical University Hospital, Taichung, Taiwan. C-HC was supported by the NIH award AAA11147 to Daria Mochly-Rosen at Stanford University, College of Medicine, U.S.A. Unique thanks to Kai-Jie Yang (Mind-Body Interface Laboratory, China Medical University Hospital, Taichung, Taiwan) and WanJung Chang (College of Chinese Medicine, College of Chinese Medicine, China Medical University) for the technical help. Conflicts of Interest: The authors declare no competing interests.
ORIGINAL ARTICLEBraz J Cardiovasc Surg 2022;37(3):335-The Protective Effect of Kaempferol Against Ischemia/Reperfusion Injury By way of Activating SIRT3 to Inhibit Oxidative StressChuang Sun1, MD; Tingting Wang1, MD; Changying Wang1, MD; Zhenyin Zhu1, MD; Xiaoni Wang1, MD; Jia Xu1, MD; Huixian An1, MDDOI: ten.APOC3 Protein supplier 21470/1678-9741-2020-ABSTRACTIntroduction: The objective of this study would be to investigate the protective impact of kaempferol against ischemia/reperfusion (IR) injury plus the underlying molecular mechanisms. Strategies: H9C2 cells had been pretreated with kaempferol for 24 hours and further insulted with IR injury. Cell vitality, reactive oxygen species (ROS) level, glutathione (GSH) level, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity, and sirtuin-3 (SIRT3), B-cell lymphoma two (Bcl2), and Bcl2-associated X protein (Bax) expressions have been evaluated. Additionally, quick interfering ribonucleic acid targeting SIRT3 was used to investigate the function of SIRT3 against IR mediated by kaempferol in vitro. IR mice models have been also established to confirm the protective effects of kaempferol on IR in vivo. Outcomes: After IR injury, H9C2 cells vitality was lowered, ROS levels, NADPH oxidase activity, and Bax expressions were improved, and GSH levels and Bcl2 expressions had been decreased. Immediately after kaempferol pretreatment, the vitality of H9C2 cells was improved.Ephrin-B2/EFNB2, Human (HEK293, His) The levels of ROS, NADPH oxidase activity, and Bax expression were decreased.PMID:24211511 Additionally, levels of GSH and Bcl2 expression have been enhanced. In addition, silencing SIRT3 attenuated the protective impact mediated by kaempferol, with increased ROS levels, NADPH oxidase activity, and Bax expression, as well as lowered GSH level and Bcl2 expression. In vivo IR model showed that kaempferol could preserve IR-damaged cardiac function. Conclusion: Kaempferol has the capability of attenuating H9C2 cells IR injury by means of activating SIRT3 to inhibit oxidative stress. Key phrases: Kaempferols. Reperfusion Injury. Sirtuin-3. Oxidative Stress.Abbreviations, acronyms symbolsBax Bcl2 CCK8 DMEM GSH IR Kae LVEF LVFS = Bcl2-associated X protein = B-cell lymphoma 2 = Cell counting kit eight = Dulbecco’s Modified Eagle’s Medium = Glutathione = Ischemia/reperfusion = Kaempferol = Left ventricular ejection fraction = Left ventricular f.
